Many analytical labs offer accelerated microbiology testing, but not all of them are using validated methods to achieve the time-saving results. Don’t let your testing fall short of FDA requirements. It’s important to understand the difference between a truly validated testing method, and merely “shortened” method.

The existing USP methods for screening for microbiological pathogens were designed as a multi-step process to detect pathogens in a variety of product types and environments.

Our new Rapid Micro Screening is designed for the nutritional products / supplement industry and has been validated to provide faster test results for both e-coli and salmonella screens.


Existing methods seek first to detect a whole host of microorganisms, and then once those cultures present, to work on identifying which ‘bad bugs” are present. By focusing on these two most common pathogens (e-colli and salmonella right from the beginning), we can boost our screening efforts significantly through a targeted approach. We use plate media designed specifically to culture these two bugs. And we are seeing results in half the time. Identification is now possible in 18-24 hours, a reduction of almost 50% from the existing methods.

The Method

The current USP 2022 method for detection of Salmonella utilizes a non-selective primary enrichment in Trypticase Soy Broth for 18 -24 hrs followed by a selective secondary enrichment in Rappaport-Vassiliadis Broth for 18 – 24 hrs.  The selective enrichment is streaked onto Hektoen Enteric and Xylose-Lysine-desoxycholate agars, both selective agars for recovery of isolates if present.  Incubation is 24 – 48 hrs.  The modified protocol utilizes a non-selective enrichment in Lactose Broth for 18 – 24 hrs.  This is followed by streaking the enrichment onto Hektoen Enteric and Xylose-Lysine-desoxycholate agars for 18 – 24 hrs.


If your lab or company could benefit from shorter TAT in these tests, talk to your Dyad Labs representative today